Edited by tbsounde2, 23 November 2009 - 10:50 PM.
Hypoxia Treatment For Cell Culture
Posted 23 November 2009 - 10:15 PM
Posted 24 November 2009 - 09:04 AM
While both DFO and CoCl2 get you stabilization of HIF1alpha protein, it is not the only thing that they do.. this means you might be chasing ghosts if you overdo that.
Hypoxia chambers (like from Billups-Rothenberg) are not so expensive and quite easy to use.. for longer term experiments I would probably use that instead of DFO/CoCl2
Posted 24 November 2009 - 11:43 AM
Posted 24 November 2009 - 03:39 PM
Posted 25 November 2009 - 04:11 AM
You could probably go 5% CO2 rest N2, but I somehow liked the defined hypoxia gas better than the complete anoxia.