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calculating IC50


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#1 roshanbernard

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Posted 23 November 2009 - 04:37 PM

Hi..can anyone tell me how can i calculate IC50..i am using some inhibitors for the enzymes...and where can i get information about how to calculate IC50..

#2 warsel

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Posted 25 November 2009 - 05:31 AM

Make a Dose-Response curve that goes from 0% to 100% inhibition.
Plot concentrations on a logarithmic scale - you should get a nice sigmoidal curve.
Pick your 50% inhibition point and find the corresponding concentration, this is your IC50 (or the log thereof, if you plotted concentrations on a log scale).

#3 roshanbernard

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Posted 25 November 2009 - 05:35 AM

Oh okie...thanks a lot.this was realy helpful..i was stuck up with this from one week...thank you..




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