3 replies to this topic

[cotchy]

Hi All, attached is a picture of cells treated with 100 ug/ml G418 for 96 h.

Does anybody know what process is occuring to the cells here? is it blebbing cause during apoptosis?

The cells were primary ovarian murine cells which were immortalised with pSV3-neo and being selected for.

However for my thesis it would be nice to have an idea what way the non-immortalised cells died after exposure to G418.

(no scale bar as it was taken with a digital camera down the eye-piece of a regular micrscope)

Thanks for any help.

Attached Files

•  G418_treated_cells.JPG   20.59K   34 downloads

[bob1]

I just see a bunch of floating cells and a few attached ones, I can't identify any particular cell undergoing apoptosis.

G418 blocks polypeptide synthesis, which should induce apoptosis...

[cotchy]

The thing is their not actually floating and adhere quite strongly to the flask, i even tapped the flask and still no movement.

i really just wanted to know if other researchers had seen similar looking cells and if they knew what was happening to them at that time

[blotted]

cotchy, on Nov 25 2009, 04:20 PM, said:

The thing is their not actually floating and adhere quite strongly to the flask, i even tapped the flask and still no movement.

i really just wanted to know if other researchers had seen similar looking cells and if they knew what was happening to them at that time

there are some papers that show apoptosis activation after treatment of G418 in many cell types.
However with this picture I don't see any special feature.