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cleaning of quartz cuvette??


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6 replies to this topic

#1 rick112

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Posted 23 November 2009 - 01:23 AM

hi

we recently found out that due to some one's bad useage of "quartz microplate (cuvette)"..have protein precipitations....is tehre any method to clean quartz cuvette???

P.S. one of my collegue told about usage of diluted NaoH for the same...i am little skeptical about it... :lol:

thanks

#2 Gerard

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Posted 23 November 2009 - 01:56 AM

hi
P.S. one of my collegue told about usage of diluted NaoH for the same...i am little skeptical about it... :lol:

thanks

For protein removal is diluted NaOH a good choise, just a few minutes soaking and several times rinsing with demi.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 Prep!

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Posted 23 November 2009 - 02:33 AM

ya u can rinse with methanol... then with pure water.. tehn witht he dilute base and again with water and store
Also found these sites might be useful... not about microplates but tells a lot about quartz!!

http://www.mindat.or...Cleaning Quartz

http://www.gamineral...z-cleaning.html
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#4 mdfenko

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Posted 23 November 2009 - 09:29 AM

we would occasionally soak our quartz cuvettes in concentrated nitric acid then rinse with dih2o.

you can also use chromic acid (or nochrom-mix if you want to avoid chromium).

Edited by mdfenko, 23 November 2009 - 09:30 AM.

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i do what i get paid to do

#5 Hielscher_Ultrasonics

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Posted 23 November 2009 - 10:29 AM

hi

we recently found out that due to some one's bad useage of "quartz microplate (cuvette)"..have protein precipitations....is tehre any method to clean quartz cuvette???

P.S. one of my collegue told about usage of diluted NaoH for the same...i am little skeptical about it... :rolleyes:

thanks


Hi Rick,

did you try ultrasonic cleaning or ultrasonically assisted NaOH-cleaning yet. We at Hielscher offer a microplate sonication system for this. You could fill your microplate with water/diluted NaOH or concentrated NaOH and the ultrasonic agitation will assist the cleaning process.

Have a nice day!

Hielscher Ultrasonics

#6 Radish

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Posted 23 November 2009 - 11:19 AM

hi

we recently found out that due to some one's bad useage of "quartz microplate (cuvette)"..have protein precipitations....is tehre any method to clean quartz cuvette???

P.S. one of my collegue told about usage of diluted NaoH for the same...i am little skeptical about it... :P

thanks


Hi Rick,

did you try ultrasonic cleaning or ultrasonically assisted NaOH-cleaning yet. We at Hielscher offer a microplate sonication system for this. You could fill your microplate with water/diluted NaOH or concentrated NaOH and the ultrasonic agitation will assist the cleaning process.

Have a nice day!

Hielscher Ultrasonics

usually we have a little container filled with concentrated nitric acid, every time we finish using the cuvettes, we would spray them with 70 % ethanol, then distilled water, and into the container. We never saw any precipitates and we avoid scratches (some people use little brushes).

#7 rick112

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Posted 24 November 2009 - 07:12 PM

Hi Hielscher_Ultrasonics,

I think one should not use ultrasonication for cleaning of quartz cuvette as the high frequencies used in these baths will damage or even shatter your cuvettes....




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