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How well do macrophages respond to DMSO?


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#1 gnarl

gnarl

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Posted 21 November 2009 - 10:54 PM

I am culturing J774.2 macrophages for the purposes of exposing them to amyloid beta and determining how that affects tnf-a.
i am using several potential inhibitors and I plan to dilute them in DMSO. I know that the ideal is below 0.5% dmso diluted with the culture medium, but are macrophages less sensitive to it or more sensitive?
Also, I am culturing outside of a co2 incubator so my medium contains HEPES buffer.
Should I dilute the DMSO with the medium containing HEPES, without HEPES, or does it not really matter?

Thanks so much!





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