I am culturing J774.2 macrophages for the purposes of exposing them to amyloid beta and determining how that affects tnf-a.
i am using several potential inhibitors and I plan to dilute them in DMSO. I know that the ideal is below 0.5% dmso diluted with the culture medium, but are macrophages less sensitive to it or more sensitive?
Also, I am culturing outside of a co2 incubator so my medium contains HEPES buffer.
Should I dilute the DMSO with the medium containing HEPES, without HEPES, or does it not really matter?
Thanks so much!
How well do macrophages respond to DMSO?
No replies to this topic