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Inflammation: Amyloid beta 25-35 vs. 1-40?


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#1 gnarl

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Posted 21 November 2009 - 10:31 PM

Hi, I'm doing a science fair project involving effect of amyloid beta on tnf-a production in macrophages, and the inhibitory effects of various compounds. I know that ab 25-35 is a lot cheaper, and takes way less time to fibrillize, so there's definitely incentive to use it.

But the focus of my project is the "various inhibitors" part, and it would be useless if amyloid beta didn't induce tnf-a production. I've seen many studies showing that 25-35 induces tnf-a, but i've also seen a few showing that it didn't have any effect by itself, only with a proven macrophage activator like LPS. There's quite a few more studies showing that 1-40 is an inflammation inducer.

Do you any of you have experience with this? A company has offered to donate several materials including amyloid beta 25-35, but I don't know if they'd still be willing to donate if I changed it to ab 1-40 (which is a good $300-400 more expensive)

Thanks for your help.

p.s. sorry about my screenname, i'm using someone else's account - i don't want you to get the idea that i think myself a "guru" when i'm only doing a science fair project :lol:

#2 forrestlin

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Posted 26 April 2010 - 06:45 PM

Hi, I'm doing a science fair project involving effect of amyloid beta on tnf-a production in macrophages, and the inhibitory effects of various compounds. I know that ab 25-35 is a lot cheaper, and takes way less time to fibrillize, so there's definitely incentive to use it.

But the focus of my project is the "various inhibitors" part, and it would be useless if amyloid beta didn't induce tnf-a production. I've seen many studies showing that 25-35 induces tnf-a, but i've also seen a few showing that it didn't have any effect by itself, only with a proven macrophage activator like LPS. There's quite a few more studies showing that 1-40 is an inflammation inducer.

Do you any of you have experience with this? A company has offered to donate several materials including amyloid beta 25-35, but I don't know if they'd still be willing to donate if I changed it to ab 1-40 (which is a good $300-400 more expensive)

Thanks for your help.

p.s. sorry about my screenname, i'm using someone else's account - i don't want you to get the idea that i think myself a "guru" when i'm only doing a science fair project :lol:


Hi,guy. Nice to meet you. I am interested in A-beta. As if A-beta 25-35 is mainly involved in mice or rats, and A-beta 1-40 or 1-42 is mainly involved in human.Maybe different A-beta have different inflammatory action on different macrophages.

Edited by forrestlin, 26 April 2010 - 06:50 PM.


#3 Vimast

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Posted 20 May 2010 - 06:53 AM

Hi, I'm doing a science fair project involving effect of amyloid beta on tnf-a production in macrophages, and the inhibitory effects of various compounds. I know that ab 25-35 is a lot cheaper, and takes way less time to fibrillize, so there's definitely incentive to use it.

But the focus of my project is the "various inhibitors" part, and it would be useless if amyloid beta didn't induce tnf-a production. I've seen many studies showing that 25-35 induces tnf-a, but i've also seen a few showing that it didn't have any effect by itself, only with a proven macrophage activator like LPS. There's quite a few more studies showing that 1-40 is an inflammation inducer.

Do you any of you have experience with this? A company has offered to donate several materials including amyloid beta 25-35, but I don't know if they'd still be willing to donate if I changed it to ab 1-40 (which is a good $300-400 more expensive)

Thanks for your help.

p.s. sorry about my screenname, i'm using someone else's account - i don't want you to get the idea that i think myself a "guru" when i'm only doing a science fair project :)


Hi,guy. Nice to meet you. I am interested in A-beta. As if A-beta 25-35 is mainly involved in mice or rats, and A-beta 1-40 or 1-42 is mainly involved in human.Maybe different A-beta have different inflammatory action on different macrophages.


Hi, I'm in a second level graduation thesis and I'm working with Abeta 1-42. A red a lot about the differences but my conclusion is that one thing is to create a protein aggregate, and in this the abeta 25-35 is sufficient, and another is to create the abeta aggregate that mimic what is present in the pathology. I also did different experiments with Abeta 1-42 and I can tell you that for the activation of microglia you need also LPS.




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