Plate shaking in ELISA
Posted 21 November 2009 - 10:20 PM
They don't mention plate shaking in their procedure, but I know for most ELISAs you need plate shaking during the incubation periods.
Does it depend on the assay kit or should I just do it anyway?
Posted 22 November 2009 - 03:58 AM
Most of the time shaking will be okay but the there is the possiblity you have to adjust times to get the right OD because shaking mostly will cause the OD to go up.
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Posted 23 November 2009 - 01:21 AM
only difference would be double incubation times in the benchtop protocol than the shaker protocol