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Single site saturation mutagenesis

Started by biolab12, Nov 20 2009 07:39 AM

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#1 biolab12

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Posted 20 November 2009 - 07:39 AM

Hi all,

I want to construct a library of mutants consisting of all 20 amino acids at a specific site within a protein by PCR. I am looking for a good strategy for primer design. Can you please help me if anyone had prior experience with this?

Cheers
bio

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