3 replies to this topic

[cardosopedro]

Hi.

When I treat cells with a compound I get an huge G2/M arrest, as assessed by flow cytometry. I want to check if the cells are arrested either in G2 or in M. How can I do this?

Is there any marker I can use to check this by immunofluorescence / flow cytometry?


Thanks for any answer.

[bob1]

You could look for microtubule formation for G2 and condensed chromosomes or similar for M

[cardosopedro]

Thanks.

Do you know how to do this? I mean, are you referring to a simple DAPI staining?

[bob1]

I guess, fix and then antibody stain for microtubules.  Condensed DNA, standard Giemsa staining should work.  Both by light microscopy or fluorescence in the case of the antibody.