4 replies to this topic

[rick112]

Hi

I would like to know if “non reducing SDS protein ladder” is available???

[Prep!]

Hey rick there are two options

1) make it yourself - which might not be feasable for all applications specialy when repeatability is needed and you make different stocks
2) a better option is that Bio-Rad provides concentrated marker solutions (low and high range) which has to be diluted with reduced sample buffer before loading. So instead of reduced you can add the non-reducing buffer and use this as the nr ladder.

Hope this helps
Best luck!!!

[rick112]

hi
thanks

well i liked the secound suggestion will look into it....

[Prep!]

i can give you the calalogue numbers if u need!!!

[mdfenko]

Pradeep Iyer, on Nov 20 2009, 01:56 AM, said:

2) a better option is that Bio-Rad provides concentrated marker solutions (low and high range) which has to be diluted with reduced sample buffer before loading. So instead of reduced you can add the non-reducing buffer and use this as the nr ladder.

you won't be able to trust the results if you don't run them as prescribed.