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Non Reducing SDS protein ladder??


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4 replies to this topic

#1 rick112

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Posted 19 November 2009 - 10:48 PM

Hi

I would like to know if “non reducing SDS protein ladder” is available???

#2 Prep!

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Posted 19 November 2009 - 10:56 PM

Hey rick there are two options

1) make it yourself - which might not be feasable for all applications specialy when repeatability is needed and you make different stocks
2) a better option is that Bio-Rad provides concentrated marker solutions (low and high range) which has to be diluted with reduced sample buffer before loading. So instead of reduced you can add the non-reducing buffer and use this as the nr ladder.

Hope this helps
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#3 rick112

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Posted 19 November 2009 - 11:00 PM

hi
thanks

well i liked the secound suggestion will look into it....

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Posted 19 November 2009 - 11:03 PM

i can give you the calalogue numbers if u need!!!
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#5 mdfenko

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Posted 20 November 2009 - 09:23 AM

2) a better option is that Bio-Rad provides concentrated marker solutions (low and high range) which has to be diluted with reduced sample buffer before loading. So instead of reduced you can add the non-reducing buffer and use this as the nr ladder.

you won't be able to trust the results if you don't run them as prescribed.
talent does what it can
genius does what it must
i do what i get paid to do




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