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I am a long time fan of your forum, so I finally decided to get out of the bushes, stop lurking and actually participate in the forum discussions
.I have been reading for a long time the advantages of the fast chip protocol when compared to the regular protocols, so I finally decided to put it in practice. However I am a little confused, do I need to do the column purification (I read in the forum arquives that you use a qiagen column to purify the DNA instead of doing the phenol chlorform extraction)? And if so, which type do you use?
To clarify my doubt:
1) crosslink and harvest cells
2)lyse cells and wash pellet
3)shear chromatine and centrifuge
4)add ab
5)centrifuge to clear aggregates
6)add protein A
7)wash beads
8)add chelex and boil
9)add proteinase K and incubate
10) boil
11) centrifuge and collect supernatant
12) column purification?
Thank you so much to all of you. Your help will be greatly appreciated.
Oh, and thank you jiro_killua I learned to do the ChIP data analysis from you
