Hello to you all!
I am a long time fan of your forum, so I finally decided to get out of the bushes, stop lurking and actually participate in the forum discussions .
I have been reading for a long time the advantages of the fast chip protocol when compared to the regular protocols, so I finally decided to put it in practice. However I am a little confused, do I need to do the column purification (I read in the forum arquives that you use a qiagen column to purify the DNA instead of doing the phenol chlorform extraction)? And if so, which type do you use?
To clarify my doubt:
1) crosslink and harvest cells
2)lyse cells and wash pellet
3)shear chromatine and centrifuge
5)centrifuge to clear aggregates
6)add protein A
8)add chelex and boil
9)add proteinase K and incubate
11) centrifuge and collect supernatant
12) column purification?
Thank you so much to all of you. Your help will be greatly appreciated.
Oh, and thank you jiro_killua I learned to do the ChIP data analysis from you
Fast ChIP + purification column
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