I need to see if I can find the ITS region in my sequences. I have sequences from amplified 18s region and it is possible that I picked up the ITS region. How do I approach this problem? I don't know what to look for in my sequences. I am new to bioinformatics, and any help is appreciated.
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#2
hobglobin
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Posted 20 November 2009 - 02:31 PM
jdgriner, on Nov 20 2009, 01:12 AM, said:
I need to see if I can find the ITS region in my sequences. I have sequences from amplified 18s region and it is possible that I picked up the ITS region. How do I approach this problem? I don't know what to look for in my sequences. I am new to bioinformatics, and any help is appreciated.
A search with BLAST might help....if the ITS region of your organism is already in the database....
One must presume that long and short arguments contribute to the same end. - Epicurus
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jdgriner
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Posted 20 November 2009 - 07:40 PM
I found out today that I need to find the paper that describes what exact regions that the SSU primers amplify. Medlin 1988 published a similar primer pair..Does anyone know what paper that is referenced in (SSU primers)?
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Adrian K
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Posted 22 November 2009 - 06:37 PM
Are you doing fungal research?
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
