I am studying the function of transcription element. The experiment I would like to do is to construct a stable cell line integrated into the genomes with my interesting promoter + reporter gene and a selectable marker gene and monitor the transactivation and repression of the reporter gene mediated by the promoter upon differentiation with time. Since the promoter might be silenced upon differention, the selection marker should be under a different promoter. For method, I would prefer virus transfection, but lipofectamine transfectiion would also work. Does anybody have idea of what vector I should look for? Thank you!
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Vector with multiple cloning site without promoter upstream and containing selec
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