home made sybr green mix for qpcr
Posted 19 November 2009 - 09:21 AM
I'd like to do my own sybr green mix with Taq gold polymerase and Sybr green 1 (purchased 10 000x in DMSO). I used to run my qPCR on CFX96, did someone do it before? Do you have any advices?
Posted 20 November 2009 - 01:06 AM
I tried a run wit Sybr at 0.5X on CFX96. There was no detection of fluorescence. I'll try again with different Sybr dilutions.
Posted 20 November 2009 - 01:30 AM
Hopefully all the other conditions are fine for smplification.. that u must have already checked!!!