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home made sybr green mix for qpcr


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#1 ciyiben

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Posted 19 November 2009 - 09:21 AM

HI
I'd like to do my own sybr green mix with Taq gold polymerase and Sybr green 1 (purchased 10 000x in DMSO). I used to run my qPCR on CFX96, did someone do it before? Do you have any advices?
Thank you

#2 ciyiben

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Posted 20 November 2009 - 01:06 AM

Hi
I tried a run wit Sybr at 0.5X on CFX96. There was no detection of fluorescence. I'll try again with different Sybr dilutions.
Bye

#3 Prep!

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Posted 20 November 2009 - 01:30 AM

Generally SYBR green is done at 1X final dye concentration.
Best luck..
Hopefully all the other conditions are fine for smplification.. that u must have already checked!!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!




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