Hello everybody,
I need to isolate specific fragments from a RACE (Rapid Amplification of cDNA Ends) reaction. Classic RACE reactions work similarly to PCR reactions, the drawback is the great amount of aspecific products that usually appear as a smear when run in an agarose gel. I would like to know if anybody knows a method to specifically precipitate (through hybridization) the specific products (alternatively I could extract them from an agarose gel, if recognizable). I would like to proceed so:
1 RACE reaction
2 hybridization with a specific DNA oligonucleotide( I already have it because of other purposes)
3 product identification and isolation
Does anybody have any idea (or protocol)?
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Selective precipitation of RACE fragments through probe hybridization
Started by marek82313, Nov 19 2009 08:53 AM
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