Only DNA ladder , No desired band in PCR
Posted 19 November 2009 - 04:00 AM
my friend with the same master mix volume, but different primer was able to get the band, is that the primer problem?
does primer expired?
Posted 19 November 2009 - 12:04 PM
I'd just try again, paying extra attention. Can you include a positive control?
And yes, primers expire, like every reagent.
Posted 23 November 2009 - 12:45 AM
Posted 23 November 2009 - 01:55 AM
Posted 23 November 2009 - 02:55 AM
this might just be is a very trivial issue unless your positive control also does not show up anything!!!