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My acrylimide gel rips.


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3 replies to this topic

#1 spellberg56

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Posted 18 November 2009 - 02:32 PM

Hello, I have been doing western blotting a lot recently. When I transfer my poly acrylimide gel to the transfer setup, it always rips and folds on itself. Is their a trick to getting it off the glass panel and on to the filter paper without falling to pieces or tearing?


Thank you.

Mike

#2 lab rat

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Posted 18 November 2009 - 02:52 PM

Hi spellberg,

I remove my gel from the glass by setting the plate gel-side down in a small tub of transfer buffer and gently working the gel off the glass into the buffer. I then use a spatula to transfer the loose gel to the membrane.

regards,

lab rat
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 bob1

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Posted 18 November 2009 - 03:15 PM

Dip whatever tool you are using to move/loosen the gel into some transfer buffer before trying to get the gel off. This will stop the gel from sticking to the tool.

#4 Feelcontraire

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Posted 19 November 2009 - 02:51 AM

Hello, I have been doing western blotting a lot recently. When I transfer my poly acrylimide gel to the transfer setup, it always rips and folds on itself. Is their a trick to getting it off the glass panel and on to the filter paper without falling to pieces or tearing?


Thank you.

Mike



What lab rat told you is the best way to go, also you can add 10% glicerine to the gel to make more stable.




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