My acrylimide gel rips.
Posted 18 November 2009 - 02:32 PM
Posted 18 November 2009 - 02:52 PM
I remove my gel from the glass by setting the plate gel-side down in a small tub of transfer buffer and gently working the gel off the glass into the buffer. I then use a spatula to transfer the loose gel to the membrane.
Posted 18 November 2009 - 03:15 PM
Posted 19 November 2009 - 02:51 AM
Hello, I have been doing western blotting a lot recently. When I transfer my poly acrylimide gel to the transfer setup, it always rips and folds on itself. Is their a trick to getting it off the glass panel and on to the filter paper without falling to pieces or tearing?
What lab rat told you is the best way to go, also you can add 10% glicerine to the gel to make more stable.