Hey Guys,
I have to start doing some experiment with this membrane protein I have. I need to express that in cells and do endocytosis experiments. After some literature search, I thought I'll use CHO cells. Any comments on how these cells are easy to handle in general, and if they are easily 'transfectable' and express recombinant proteins properly.
Thanks in advance for your time!
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Posted 26 November 2009 - 07:46 AM
jangajarn, on Nov 19 2009, 04:56 AM, said:
Hey Guys,
I have to start doing some experiment with this membrane protein I have. I need to express that in cells and do endocytosis experiments. After some literature search, I thought I'll use CHO cells. Any comments on how these cells are easy to handle in general, and if they are easily 'transfectable' and express recombinant proteins properly.
Thanks in advance for your time!
I have to start doing some experiment with this membrane protein I have. I need to express that in cells and do endocytosis experiments. After some literature search, I thought I'll use CHO cells. Any comments on how these cells are easy to handle in general, and if they are easily 'transfectable' and express recombinant proteins properly.
Thanks in advance for your time!
Hello~~
I am using a CHO-K1 cell line for toxicity study, I think CHO is very easy to handle, fast growth (doubling time around 12~14 hrs), I don't think is it a good thing for you because you need to spilt 2 to 3 times a week. Media are very comment, either DMEM or F-12 or both, 10%FBS gives very good result, so even I don't need to do recombinant protein but I believe you will get a pretty good result.
Hope this help.
