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reference genes for qPCR


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3 replies to this topic

#1 susanna

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Posted 18 November 2009 - 08:33 AM

Hi,

i'm doing qPCR and know that you have to take some normalization genes in each run.
I wondered which genes, are usually used.
Do somebody has some experience with this? And which genes, did you use?

greetz

#2 Vini

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Posted 18 November 2009 - 08:52 AM

Hi,

i'm doing qPCR and know that you have to take some normalization genes in each run.
I wondered which genes, are usually used.
Do somebody has some experience with this? And which genes, did you use?

greetz



Hi

you should take a housekeeping gene/constitutive gene for normalization purposes...Genes which would remain unaffected even with different conditions used. ppl use actin, GAPDH etc....i use a mitochondrial ribosomal subunit......depends on the system that you are working on....

Best....

#3 unity

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Posted 19 November 2009 - 09:58 AM

depends on the system that you are working on....

True.
Actin usually works fine but it's not a good choice if your RNA samples weren't treated with DNase before.
Besides, pbgd and h-mrpl, from those that I know.

#4 susanna

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Posted 20 November 2009 - 02:17 AM

depends on the system that you are working on....

True.
Actin usually works fine but it's not a good choice if your RNA samples weren't treated with DNase before.
Besides, pbgd and h-mrpl, from those that I know.


yes, i'm using DNase before.
Thanks for the reply!




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