Hi,
i'm doing qPCR and know that you have to take some normalization genes in each run.
I wondered which genes, are usually used.
Do somebody has some experience with this? And which genes, did you use?
greetz
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3 replies to this topic
#2
Vini
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Posted 18 November 2009 - 08:52 AM
susanna, on Nov 18 2009, 11:03 PM, said:
Hi,
i'm doing qPCR and know that you have to take some normalization genes in each run.
I wondered which genes, are usually used.
Do somebody has some experience with this? And which genes, did you use?
greetz
i'm doing qPCR and know that you have to take some normalization genes in each run.
I wondered which genes, are usually used.
Do somebody has some experience with this? And which genes, did you use?
greetz
Hi
you should take a housekeeping gene/constitutive gene for normalization purposes...Genes which would remain unaffected even with different conditions used. ppl use actin, GAPDH etc....i use a mitochondrial ribosomal subunit......depends on the system that you are working on....
Best....
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Posted 19 November 2009 - 09:58 AM
DRN, on Nov 18 2009, 05:52 PM, said:
depends on the system that you are working on....
Actin usually works fine but it's not a good choice if your RNA samples weren't treated with DNase before.
Besides, pbgd and h-mrpl, from those that I know.
#4
susanna
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Posted 20 November 2009 - 02:17 AM
unity, on Nov 19 2009, 10:58 AM, said:
DRN, on Nov 18 2009, 05:52 PM, said:
depends on the system that you are working on....
Actin usually works fine but it's not a good choice if your RNA samples weren't treated with DNase before.
Besides, pbgd and h-mrpl, from those that I know.
yes, i'm using DNase before.
Thanks for the reply!
