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Two stop codons


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#1 Shaggy

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Posted 18 November 2009 - 02:30 AM

I have a pcr product with a start and stop codon....its inserted into a pCMV vector that has a signal sequence at the c terminal end...now if i express it will the expression stop at the stop codon in the gen or at the stop codon after the signal sequence.... I want the signal sequence to fuse with my protein.......

Pls help asap
ASM

#2 dpo

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Posted 18 November 2009 - 02:50 AM

translation will stop at the first stop codon the ribosome encounters, so if your PCR product contains a stop codon at the end, translation will stop there and you will not get a fusion with your signal sequence in the vector.




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