Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

protocol of glycine elution gradient


  • Please log in to reply
2 replies to this topic

#1 shm267

shm267

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 18 November 2009 - 12:57 AM

Hello all, I am going to elute my protein complex from the antibody covalently binding to the proteinA beads. one protocol recommended to use glycine elution gradient. I am not quite familiar with this techniques, does anybody have a protocol? thanks :rolleyes:

#2 Prep!

Prep!

    Am I me???!!!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 517 posts
4
Neutral

Posted 18 November 2009 - 01:04 AM

http://www.piercenet...6BF3E92#buffers

might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#3 shm267

shm267

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 18 November 2009 - 01:15 AM

http://www.piercenet...6BF3E92#buffers

might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute

thank you for your help.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.