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#1
shm267
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Posted 18 November 2009 - 12:57 AM
Hello all, I am going to elute my protein complex from the antibody covalently binding to the proteinA beads. one protocol recommended to use glycine elution gradient. I am not quite familiar with this techniques, does anybody have a protocol? thanks
#2
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Posted 18 November 2009 - 01:04 AM
http://www.piercenet...6BF3E92#buffers
might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
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#3
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Posted 18 November 2009 - 01:15 AM
Pradeep Iyer, on Nov 18 2009, 01:04 AM, said:
http://www.piercenet...6BF3E92#buffers
might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
