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protocol of glycine elution gradient - Biochemistry - BioForum

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protocol of glycine elution gradient


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2 replies to this topic

#1 shm267

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Posted 18 November 2009 - 12:57 AM

Hello all, I am going to elute my protein complex from the antibody covalently binding to the proteinA beads. one protocol recommended to use glycine elution gradient. I am not quite familiar with this techniques, does anybody have a protocol? thanks :rolleyes:

#2 Prep!

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Posted 18 November 2009 - 01:04 AM

http://www.piercenet...6BF3E92#buffers

might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
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#3 shm267

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Posted 18 November 2009 - 01:15 AM

http://www.piercenet...6BF3E92#buffers

might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute

thank you for your help.




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