Hi Everyone..!
I want to search whether a particular inverted repeat sequence present in the promoter region of E.coli gene is also present in other species of bacteria. Since it is just a 20bp long region, I dont know how to use NCBI blast option sad.gif guide me pls rolleyes.gif . I thank all of you..
Abhay
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2 replies to this topic
#2
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Epigenetist
Posted 17 November 2009 - 10:32 PM
You can read this guide from NCBI http://blast.ncbi.nlm.nih.gov/Blast.cgi?CM..._TYPE=FAQ#Short
Basically, you need to set a high value for "expect", turn off filter, and set a small window size.
Basically, you need to set a high value for "expect", turn off filter, and set a small window size.
#3
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Posted 17 November 2009 - 11:50 PM
pcrman, on Nov 18 2009, 12:02 PM, said:
You can read this guide from NCBI http://blast.ncbi.nlm.nih.gov/Blast.cgi?CM..._TYPE=FAQ#Short
Basically, you need to set a high value for "expect", turn off filter, and set a small window size.
Basically, you need to set a high value for "expect", turn off filter, and set a small window size.
Thank you very much for timely reply. I shall go through.
