Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

histological staining for plant cell wall components

  • Please log in to reply
1 reply to this topic

#1 Moondust



  • Active Members
  • Pip
  • 6 posts

Posted 17 November 2009 - 05:59 PM

Hi all,

I would like to stain cellulose and lignin in the cell wall and wonder what kinda stains are most suitable for fixed tissues.
I was told that for lignin with phloroglucinol, fresh tissues are needed. Are there any other stains that can be used to stain lignin of fixed tissues? I have currently fixed them in ethanol and acetic acid.

I've seen others who added formalin besides EtOH and acetic acid. Any idea how formalin fixes the cell..eg what's the biochemistry behind it?

If anyone has a list of stains/dyes that can differentiate the different component of cell walls (particularly cellulose) and whether fresh/ fixed tissues can be used with the respective stains, could you please share it wi th me? Many thanks! :)

#2 toejam


    Guitar Hero

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 159 posts

Posted 01 December 2009 - 09:40 AM


i'd recommend you the book "plant microtechnique and microscopy" by steven e ruzin. it's got tables of stains and many different protocols and tips for microscopy.
"When there's no more room in hell the dead will walk the Earth"

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.