2 replies to this topic

[Maddie]

Hi everyone

I need to make sure my silanized glass tubes are DNA free. What can I do? Cross-linker? I'm not sure I could use bleach. What do you think?
I just need DNA free tubes where DNA won't bind to the tube.

Thanks for your help.

Maddie

[Feelcontraire]

Maddie, on Nov 17 2009, 03:55 PM, said:

Hi everyone

I need to make sure my silanized glass tubes are DNA free. What can I do? Cross-linker? I'm not sure I could use bleach. What do you think?
I just need DNA free tubes where DNA won't bind to the tube.

Thanks for your help.

Maddie

You can touch the inside walls of the tubes with a DNA adhering material (simply electrostatically charged plastic). Then stain the DNA, and compare one that touched the inside walls and one that didn't under the fluorescence microscope.

Take a look at this: http://www.biotechniques.com/Biotechniques...ques-92159.html

To avoid electrostatic interaction of the dna with the tube and increase it solubility (i.e. clean it) you can use high Nacl concentrations and/or detergents like triton.

Edited by Feelcontraire, 18 November 2009 - 03:00 AM.

[Maddie]

Hi Feelcontraire,

I finally had the time to read the Biotechnique paper. Thanks a lot for that.
I must say I had a good laugh when reading it, especially the: "Special safety measures should be taken during the preparation of the sulfuric acid/hydrogen peroxide solution (piranha solution)" .
Hmm not really looking forward to working with an explosive piranha solution.
I'd rather buy "ready to go" silanized glass bottle:
http://www.fishersci.com/wps/portal/SEARCH...AdvanceOptions=

and THEN clean it. Is that possible? I just need to make sure the contaminating human DNA that may be in the glass bottles won't be amplifiable.