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Treatments to clean silanized glass tubes


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#1 Maddie

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Posted 17 November 2009 - 03:55 PM

Hi everyone

I need to make sure my silanized glass tubes are DNA free. What can I do? Cross-linker? I'm not sure I could use bleach. What do you think?
I just need DNA free tubes where DNA won't bind to the tube.

Thanks for your help.

Maddie
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein

#2 Feelcontraire

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Posted 18 November 2009 - 02:54 AM

Hi everyone

I need to make sure my silanized glass tubes are DNA free. What can I do? Cross-linker? I'm not sure I could use bleach. What do you think?
I just need DNA free tubes where DNA won't bind to the tube.

Thanks for your help.

Maddie


You can touch the inside walls of the tubes with a DNA adhering material (simply electrostatically charged plastic). Then stain the DNA, and compare one that touched the inside walls and one that didn't under the fluorescence microscope.

Take a look at this: http://www.biotechni...ques-92159.html

To avoid electrostatic interaction of the dna with the tube and increase it solubility (i.e. clean it) you can use high Nacl concentrations and/or detergents like triton.

Edited by Feelcontraire, 18 November 2009 - 03:00 AM.


#3 Maddie

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Posted 19 November 2009 - 08:20 AM

Hi Feelcontraire,

I finally had the time to read the Biotechnique paper. Thanks a lot for that.
I must say I had a good laugh when reading it, especially the: "Special safety measures should be taken during the preparation of the sulfuric acid/hydrogen peroxide solution (piranha solution)" :P .
Hmm not really looking forward to working with an explosive piranha solution. :lol:
I'd rather buy "ready to go" silanized glass bottle:
http://www.fishersci...AdvanceOptions=

and THEN clean it. Is that possible? I just need to make sure the contaminating human DNA that may be in the glass bottles won't be amplifiable.
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein




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