Liquid nitrogen problem
Posted 17 November 2009 - 12:16 PM
Posted 17 November 2009 - 01:00 PM
If your tank "suddenly becomes almost empty" without reason, you should replace the tank. If it was open for a significantly long time and the LN2 is lost by evaporation...well, someone should consult the inventory before searching for vials and keep the tank covered when they are not actively removing something.
If you store your cells in the vapor phase to begin with, there is little danger. If you store in the liquid phase, there may be some loss of viability. I should think you can add 1/2 LN2 without destroying your cell cultures, as we have done this several times.
Posted 18 November 2009 - 06:48 AM