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Does anybody have experience in culturing human primary B cells?

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#1 TracyDuke



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Posted 17 November 2009 - 12:00 PM

I have read a lot of literatures and tried different combinations of cytokins and stimulations but my B cells keep dying. By day 4 of culture, almost all cells die. Can anybody who has experience in B cell culture give me some advices? Thank you!

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