Posted 17 November 2009 - 11:48 AM
Posted 11 February 2010 - 09:31 PM
My 2 ct.
Dave- Dilyx Biotechnologies
Hi everybody. After purification of a membrane fraction, I have measured the absorbance at 215nm (peptide bond absorption) and at 280nm (absorption by aromatic amino acids (tyrosine, tryptophan and phenylalanine)). Now, at 215 nm the peak corresponding to the monomer is sharper than at 280 and the peak corresponding to the aggregates almost disappears. THe preparation os identical in both measurements. Any hint on why this is happening? And which measurement at this point is most reliable? Thanks a lot!