Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cell culture media


  • Please log in to reply
1 reply to this topic

#1 smbio

smbio

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 17 November 2009 - 10:26 AM

Has anyone observed changes in cell morphology (cancer cells) when cultured in DMEM and DMEM/F-12? Is there any reason for this? Thanks!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,728 posts
399
Excellent

Posted 17 November 2009 - 03:43 PM

There might not be morphological changes on culturing in different media, but there could be physiological or gene expression changes that you wouldn't see. It is possible to wean cells onto new mediums if you want to. All you need to do is mix the mediums at different ratios over several passages, so that the cells can adapt.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.