Posted 17 November 2009 - 09:52 AM
I'm starting a new lab and been having problems with a western blot. I have tryed everything and I have no signal!
People here told me once the lab fridge went out of current a whole weekend and all antibodies are there! My boss says nothing happened, that abs can resit that, but now I'm not so sure (luminol was there too by the way).
I did dot blot just with my secondary on PVDF membrain, (1:10 and 1:10 000) and saw nothing =(
What do you think about it?
Posted 17 November 2009 - 03:40 PM
The luminol should be fine, though the H2O2 is not so stable.
Posted 20 November 2009 - 03:51 PM