I´ve a problem with my real time PCR Rotor Gene 6000.
When I use Qiagen SYBR Green Master Mix, the machine works and it read the fluorescense; but when I use Fermentas SYBR Green Master Mix, this machine can not read the fluorescense. It seems to be lower than Qiagen Master Mix...
I´ve tried to change the gain (manually and automatically) for these master mix too, but still doesn´t work with Fermentas SYBR Green.
I´ve changed the master mix three times, but still doesn´t work.
I don´t know what it is... Why did Qiagen SYBR Green work and Fermentas didn´t?
Thank you for your help.
Edited by deilepaita, 17 November 2009 - 06:25 AM.