3 replies to this topic

[L3mOn]

I've always heard about putting in ~1cell/well in a 96-well or 384-well microplate. How can you really be sure that the well really contain 1 cell?

[Feelcontraire]

autumnhush, on Nov 17 2009, 02:32 AM, said:

I've always heard about putting in ~1cell/well in a 96-well or 384-well microplate. How can you really be sure that the well really contain 1 cell?

Well, you can try to check it under the light microscope with the shutter closed or under fluorescence microscope.

[L3mOn]

Oh, is that the only way? Is there like some special equipment for such tasks? Cuz 384-wells in 1 plate is a lot...and I don't just have one plate to verify...lol

[Prep!]

i ve never heard of people verifying if there is 1 cell/well. The addition is based on the cell count and subsequent dilution so that the well has a cell/well!!!
i ve never done this hands though!!!