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High Throughput Microplate Screening


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#1 L3mOn

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Posted 17 November 2009 - 02:32 AM

I've always heard about putting in ~1cell/well in a 96-well or 384-well microplate. How can you really be sure that the well really contain 1 cell?

#2 Feelcontraire

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Posted 18 November 2009 - 03:26 AM

I've always heard about putting in ~1cell/well in a 96-well or 384-well microplate. How can you really be sure that the well really contain 1 cell?


Well, you can try to check it under the light microscope with the shutter closed or under fluorescence microscope.

#3 L3mOn

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Posted 19 November 2009 - 06:19 PM

Oh, is that the only way? Is there like some special equipment for such tasks? Cuz 384-wells in 1 plate is a lot...and I don't just have one plate to verify...lol

#4 Prep!

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Posted 19 November 2009 - 08:10 PM

i ve never heard of people verifying if there is 1 cell/well. The addition is based on the cell count and subsequent dilution so that the well has a cell/well!!!
i ve never done this hands though!!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!




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