Posted 06 May 2001 - 09:00 PM
Dirty DNA will give a lot of junk if you try to PCR it. If you have many bands or smears, gel purify it first. The blender will certainly shear large chromosomal DNA, so that may be a bad thing to do. Also, don't use too much as a template--I generally use 10-50 ng as template (in bacteria and yeast--in higher eukarotes, I wouldn't go much higher than 200 ng--do more cycles if you need more product).