Posted 13 March 2002 - 01:33 AM
Posted 15 March 2002 - 11:20 AM
I am trying this technique right now, so I have no results no problems. I would like to know about your method so I could compare with mine.
Posted 15 March 2002 - 04:33 PM
the complexity depends on hoe clever u r. idea is to do 2 sets of pcr's and then use the pcr prdt to do the 3rd pcr. and 3 pcr is the opverlap pcr where it will yield deleted insert or etc..
there are system u can buy like pfu/taq mixture that ui can use in TA cloning for the internmediate steps.