hi all,
how does everyone collect serum from blood.I used the BD microtainer serum separator tubes to collect the serum from mice,but after doing the electrophoresis there seems to be fibrinogen in it.i take the blood from the mice using capillary tubes and then flush it into the SStubes.i allow the tubes to stand for more than an hour before doing the centrifuge.i then aspirate the serum that usually stands above the gel.
Thanks
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Posted 18 November 2009 - 03:46 AM
serum, on Nov 16 2009, 07:34 PM, said:
hi all,
how does everyone collect serum from blood.I used the BD microtainer serum separator tubes to collect the serum from mice,but after doing the electrophoresis there seems to be fibrinogen in it.i take the blood from the mice using capillary tubes and then flush it into the SStubes.i allow the tubes to stand for more than an hour before doing the centrifuge.i then aspirate the serum that usually stands above the gel.
Thanks
how does everyone collect serum from blood.I used the BD microtainer serum separator tubes to collect the serum from mice,but after doing the electrophoresis there seems to be fibrinogen in it.i take the blood from the mice using capillary tubes and then flush it into the SStubes.i allow the tubes to stand for more than an hour before doing the centrifuge.i then aspirate the serum that usually stands above the gel.
Thanks
Well, I suppose your issue comes from fibrinogen clothing more than from fibrinogen itself. If I remember well you should use EDTA to avoid this effect. Have you checked if your tubes have EDTA? Also check temperature.
Take a look at: http://www.sciencedirect.com/science?_ob=A...2ffbc57ba13b5da
