Posted 16 November 2009 - 03:11 PM
Posted 16 November 2009 - 03:56 PM
Posted 16 November 2009 - 10:35 PM
Well, more details would be needed, but in an IDEAL situation, your single mutants would have a unique restriction site in between them, and one on one side or the other, and the double mutants could be created by simply switching fragments....it would require cloning obviously, but it would be extremely easy and no sequencing would be required (assuming your single mutants are already sequenced). However, more details are needed. Warren..
Posted 16 November 2009 - 10:56 PM