3 replies to this topic

[SYC1234]

Hi All,

I am looking for serum substitutes for FBS to grow 293-T cells. I found some that are for FCS, but a commercial one for FBS..not found yet! !

Does anyone know or can suggest which company has serum substitutes for FBS.

Thanks a bunch.

Warm Regards,

Sonia

[bob1]

Invitrogen is starting to produce serum free culture systems, you could try them.

[klinmed]

SYC1234, on Nov 16 2009, 10:08 PM, said:

Hi All,

I am looking for serum substitutes for FBS to grow 293-T cells. I found some that are for FCS, but a commercial one for FBS..not found yet! !

Does anyone know or can suggest which company has serum substitutes for FBS.

Thanks a bunch.

Warm Regards,

Sonia

Fetal calf serum (FCS) and fetal bovine serum (FBS) are different names for the same product!  It is obtained by cardiac-puncture of bovine fetuses (cow fetus=unborn calf) following slaughter of the cow.
Not a very nice product, but there you are.

[pesji]

SYC1234, on Nov 16 2009, 01:08 PM, said:

Hi All,

I am looking for serum substitutes for FBS to grow 293-T cells. I found some that are for FCS, but a commercial one for FBS..not found yet! !

Does anyone know or can suggest which company has serum substitutes for FBS.

Thanks a bunch.

Warm Regards,

Sonia

Hi Sonia,

If you want to go away from FBS you can try Primatone extract which for certain cells works good (certain hybridoma lines)