Different types/Brands of BSA?
Posted 16 November 2009 - 12:13 PM
I have been having difficulties with PCR inhibition due to plant compounds and have attempted using BSA to no avail. A colleague of mine mentioned he had used many brands of BSA before and some had worked and some didn't. Are there different types of BSA? If not why would they differ so much by brand? What type of BSA would be best for preventing plant compound inhibition?
Posted 16 November 2009 - 06:48 PM
You will also need to make sure that it is not an acetylated BSA. Acetylation kills nucleases, nickases, and proteases but also inhibits PCR reactions
Posted 19 November 2009 - 12:27 PM
Sigma has very good non acetylated BSA (liquid or powder).