Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

could FLAG-tagged protein be used in the Luc assay?


  • Please log in to reply
1 reply to this topic

#1 gyma

gyma

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 144 posts
2
Neutral

Posted 16 November 2009 - 05:11 AM

Hi, everyone. I have several expression plasmids which are all flag-tagged and I have already used them in my Luc assay. I am wondering if FLAG affects the result? Is there anyone else ever using a tagged plasmid in the Luc assay? Thanks in advance.

#2 laurequillo

laurequillo

    The Goddamn Batman!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 265 posts
1
Neutral

Posted 07 December 2009 - 09:57 PM

Hi there,

they should not affect your resulst at all. I do it eveyday.

But just to be sure it would be good to check some untagged vector or some vectors with a different tag. The reason is not because flag-tag could affect the luc assay, the reason is because sometimes different tags or different location of the tag (N vs C terminal tags) can affect the activity of the protein. So, if you have any doubt, it would be good to check the same protein with different tags, or even better the untagged protein to see if they have the same behaviour.
"He must be very ignorant for he answers every question he is asked" Voltaire

"This is SPARTA!"

"Im the goddamn batman"




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.