Removal of Methionine in E.coli

Started by pET, Nov 16 2009 02:20 AM

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#1 pET

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Posted 16 November 2009 - 02:20 AM

Dear all
I am expressing mammalian proteins in E.coli. For downstream applications its important to know now if the Start-Methionine is removed. My inserts are inp pET / BL21 and I use the vector-encoded ATG.
Can I rely that during protein processing the Methionine is removed ?
pET