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rna from plasmid


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#1 soymilk14

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Posted 16 November 2009 - 01:19 AM

hello everyone,

i am still new in this field and i would like to ask from which link or reference materials can i know the procedure and concept of synthesizing biotinylated rna from plasmid with the gene of interest into insert to the plasmid.

thank you so much.

#2 zhongmindai

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Posted 16 November 2009 - 02:30 AM

The plasmid you use must have a T7 or SP6 or T3 RNA polymerase recognition site. So you can use one of these RNA polymerase to synthesis the RNA of your interested gene in vitro. Using the Biotin-labled UTP instead of UTP in the transcription reaction.
A lot of companies have kit for the in vitro transcription of biotinated RNA. The url listed below is just obtained by a google search.
https://www.roche-ap...f/ISH_54-57.pdf
http://www.epibio.com/item.asp?ID=519
Zhong-Min Dai




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