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[soymilk14]

hello everyone,

i am still new in this field and i would like to ask from which link or reference materials can i know the procedure and concept of synthesizing biotinylated rna from plasmid with the gene of interest into insert to the plasmid.

thank you so much.

[zhongmindai]

The plasmid you use must have a T7 or SP6 or T3 RNA polymerase recognition site. So you can use one of these RNA polymerase to synthesis the RNA of your interested gene in vitro. Using the Biotin-labled UTP instead of UTP in the transcription reaction.
A lot of companies have kit for the in vitro transcription of biotinated RNA. The url listed below is just obtained by a google search.
https://www.roche-applied-science.com/PROD_...f/ISH_54-57.pdf
http://www.epibio.com/item.asp?ID=519