Opinions on Fermentas DreamTaq Green PCR Master Mix?
Posted 16 November 2009 - 01:02 AM
Posted 28 November 2009 - 11:08 AM
FYI, for anyone who's interested, I ended up buying some, since this is the least expensive green master mix that my uni's biostore stocks (and my remaining green master mix from Promega "ran out" after an unfortunate incident involving an open tube, gravity, and my pants.) Anyway, I use colored 0.2 ml pcr tubes from DOT since I have a lot of samples to screen and colorcoding groups helps me keep them straight. The Fermentas mix worked fine, *EXCEPT* that the product in my little red tubes turned pink and then had no product on the gel of any sort! The product from the orange, yellow, blue, and purple tubes was fine though. Weird, right? I'm going to write to Fermentas and compain, but... well, I guess you get what you pay for! (Although the Promega GoTaq green master mix is actually about the same price!)
Posted 28 November 2009 - 11:29 AM
Posted 30 November 2009 - 04:13 PM
Actually, it turned out that the problem was with the samples -- same thing happened with the Promega mix, in a different tube color! Something clearly went wrong with those extractions...
As for the colored tubes... well, when they run out I will probably buy regular clear ones, just as a matter of cost. But I like the colored tubes, all my pcr product looks so happy and cheerful in the rack.