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20x SSC


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#1 ashpra

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Posted 15 November 2009 - 11:49 PM

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance

#2 Prep!

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Posted 16 November 2009 - 12:35 AM

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance



well i ve never done a norhern blot but my concept says higher salt.. better diffusion.. lesser time for blotting!!!
Support bacteria - They are the only culture some people have!!!
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#3 ashpra

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Posted 18 November 2009 - 12:13 PM

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance



well i ve never done a norhern blot but my concept says higher salt.. better diffusion.. lesser time for blotting!!!


thank u for the reply but I think your answer defies the principle of diffusion...

#4 Prep!

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Posted 18 November 2009 - 07:15 PM

ooooops i meant capillary.. sorry for tat!!!
Support bacteria - They are the only culture some people have!!!
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