3 replies to this topic

[ashpra]

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance

[Prep!]

ashpra, on Nov 16 2009, 01:19 PM, said:

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance

well i ve never done a norhern blot but my concept says higher salt.. better diffusion.. lesser time for blotting!!!

[ashpra]

Pradeep Iyer, on Nov 16 2009, 12:35 AM, said:

ashpra, on Nov 16 2009, 01:19 PM, said:

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance

well i ve never done a norhern blot but my concept says higher salt.. better diffusion.. lesser time for blotting!!!

thank u for the reply but I think your answer defies the principle of diffusion...

[Prep!]

ooooops i meant capillary.. sorry for tat!!!