Ligation Time and Temp
Posted 14 November 2009 - 08:23 AM
I amplifed my 700bp fragment with PCR and now I need to ligate a 700bp fragment to a 6kb vector using xhoI and NdeI sites. Would 90 min at RT or 90 min at 4C work?
Also, do i need to gel purify my pcr reaction before ligation? Will it work if i just use my pcr reaction directly or do pcr purify?
Posted 14 November 2009 - 10:15 AM
Posted 14 November 2009 - 12:41 PM
Posted 15 November 2009 - 03:09 PM
Posted 15 November 2009 - 06:14 PM