Incomplete denaturing with 8M urea?
Posted 13 November 2009 - 02:31 PM
Posted 18 November 2009 - 12:53 PM
genius does what it must
i do what i get paid to do
Posted 01 December 2009 - 02:34 AM
2. + SDS
3. +SDS+beta-mercap+boil for 10min (like what u do in SDS-PAGE)
I think boiling will destroy the native structure, if it do not totally destroy the protein.
Posted 03 December 2009 - 08:00 AM