Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Transfecting suspension cells

  • Please log in to reply
No replies to this topic

#1 hvangapandu



  • Members
  • Pip
  • 3 posts

Posted 13 November 2009 - 12:24 PM

I transfected Colo205 that grow in suspension. I realized these cells are very hard to transfect. Transfection efficiency is as low as 0.1%. Has any one transfected these cells before? After transfection in serum free medium , to get rid of the lipid (transfecting reagent), is it advisable to spin down the cells and resuspend them in regular media, or should I directly add media after 5-6 hrs of transfection?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.