Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cell lysis buffer


  • Please log in to reply
1 reply to this topic

#1 aspergillus

aspergillus

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 13 November 2009 - 12:02 PM

Hi, I am planning to make cell lysis buffer (10mM Tris-HCl,100mM EDTA,2% SDS) for genomic dna extraction and was wondering if I need to autoclave it after I make it? I have always been using cell lysis buffer that comes with extraction kits and it's the first time I'll be using in house buffer.
Thanks in advance

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,236 posts
336
Excellent

Posted 15 November 2009 - 03:40 PM

Don't autoclave SDS containing solutions, it will foam like crazy causing you to lose a lot of the buffer. Also it will denature the SDS, not that this affects its ability to act as a detergent.

You could filter it, though with the SDS, I would be surprised if anything will grow in it.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.