Hi, I am planning to make cell lysis buffer (10mM Tris-HCl,100mM EDTA,2% SDS) for genomic dna extraction and was wondering if I need to autoclave it after I make it? I have always been using cell lysis buffer that comes with extraction kits and it's the first time I'll be using in house buffer.
Thanks in advance
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bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 15 November 2009 - 03:40 PM
Don't autoclave SDS containing solutions, it will foam like crazy causing you to lose a lot of the buffer. Also it will denature the SDS, not that this affects its ability to act as a detergent.
You could filter it, though with the SDS, I would be surprised if anything will grow in it.
You could filter it, though with the SDS, I would be surprised if anything will grow in it.
