Posted 13 November 2009 - 08:00 AM
I have a little question.:
if you design expression-primers using primer-blast or another primerdesign software, is it necessary that the primers are separated by at least one intron on the corresponding genomic DNA? In primer-blast you can click on this function, but then you find less primers...
Posted 13 November 2009 - 10:04 AM
Posted 14 November 2009 - 10:19 AM
Posted 17 November 2009 - 06:14 AM
But i thought that, if you click on: "primer has to span exon-exon boarders", that this would automatically exclude gDNA, cause only mRNA has exon-exon boarders. While genomic DNA always has an intron between this exons. and because of this, it wasnt necessary to click: "primers has to be separated by an intron"