Posted 13 November 2009 - 07:50 AM
Posted 15 November 2009 - 02:51 PM
Unless you have a protocol for growth/proliferation (conversion to a cell line), you are going to have problems with cell division. Of course with each doubling/passage, all cells "age".
Posted 16 November 2009 - 11:36 AM
Exactly what do you mean by the cells "aging"? If you want to know whether cells isolated from a young rat and then cultured for a while will be equivalent to cells freshly isolated from an older rat, then no.
What cell type are you interested in?
then heaven will be yours, before you meet your end