Hi I was wondering if I prepare prmary cells from young rat. does the cell will age as well in a dish?
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#2
amelia417
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Posted 15 November 2009 - 02:51 PM
ulujm, on Nov 13 2009, 10:50 AM, said:
Hi I was wondering if I prepare prmary cells from young rat. does the cell will age as well in a dish?
Unless you have a protocol for growth/proliferation (conversion to a cell line), you are going to have problems with cell division. Of course with each doubling/passage, all cells "age".
#3
gfischer
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Posted 16 November 2009 - 11:36 AM
ulujm, on Nov 13 2009, 09:50 AM, said:
Hi I was wondering if I prepare prmary cells from young rat. does the cell will age as well in a dish?
Exactly what do you mean by the cells "aging"? If you want to know whether cells isolated from a young rat and then cultured for a while will be equivalent to cells freshly isolated from an older rat, then no.
What cell type are you interested in?
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